Biofilm formation is an important virulence factor which is controlled by complex regulatory circuits in . In this work, a biofilm hyper-producing strain, P2-7, was selected from a collection of transposon insertion mutants in which the gene was disrupted. was predicted as a putative LysR-type regulator. Analyses showed that it was involved in early biofilm formation, mature biofilm development, and colony morphology. Quantitative measurements revealed that repressed biosynthesis of extracellular polysaccharides (alginate, psl and pel). Furthermore, it was observed that was self-regulated, highly expressed in the early phase of biofilm development, and subject to the negative regulation by a biofilm synthesis regulator SrpA that binds directly to the gene promoter. Collectively, this study proposes that PA2121 is a novel biofilm synthesis repressor (BsrA) in .
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