Taking advantage of the compelling properties of d-penicillamine (d-PA) combined with copper, a method for the sensitive and selective determination of d-PA was established using copper nanocluster (Cu NC)-based fluorescence enhancement. d-PA molecules containing a thiol compound showed a strong tendency to combine with the surface of Cu NCs, causing the re-dispersion of nanoclusters and therefore fluorescence intensity was enhanced. Fluorescence enhancement efficiency of Cu NCs induced by d-PA was linear, with the d-PA concentration varying from 0.6-30 μg ml (R = 0.9952) and with a detection limit of 0.54 μg ml . d-PA content in human urine samples was detected with recoveries of 104.8-112.99%. Fluorescence-enhanced determination of d-PA using Cu NCs was established for the first time and this rapid, easy and sensitive method should attract much attention for this application.
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