Micronuclei Formation Analysis After Ionizing Radiation.

Micronuclei are formed by broken chromosome fragments or chromosomes, which were not appropriately separated into the daughter cells' nuclei after division. The appearance of micronuclei is typically a sign of genotoxic events. Majority of micronuclei are formed by broken acentric fragments, but some micronuclei are formed by centric chromosome fragments which were not appropriately separated to daughter cells' nuclei. Because researchers only need to measure visible micronuclei in binucleated cells, micronuclei analysis is much easier than metaphase chromosome aberration analysis discussed in the previous chapter. This method does not require professional training compared to metaphase chromosome aberration analysis. In addition, one can analyze many samples in a relatively short time. Not only ionizing radiation, but other genotoxic stress also induces micronuclei formation. The background frequency of micronuclei is noticeably higher than chromosome aberrations. But researchers can easily analyze 300-1000 binucleated cells per data point to obtain statistically significant differences of irradiated samples. In this chapter, we will discuss the advantages and preparation of micronuclei samples.