Development of simple diffuse optical metabolic spectroscopy for tissue metabolism measurement.

In the field of biomedicine, there are optical systems that provide the tissue metabolic rate of oxygen consumption (tMRO) by the simultaneous measurement of blood flow and oxygenation level. However, current optical systems are costly and require complex optical alignments, which are inconvenient for clinical applications. Therefore, in this study, we developed a simple diffuse optical metabolic spectroscopy system by combining a broadband light source and a laser and by sharing a spectrometer as a detector for both diffuse optical spectroscopy and diffuse speckle contrast analysis. This system simultaneously measures blood flow, volume, and oxygenation in a simple and cost-effective manner. The system response to flow is demonstrated through the flow phantom experiments. The results of the experiments show that flow response is in the range 0~0.9 ml/min, with a resolution better than 0.1 ml/min. During the blood phantom study, the blood volume fraction increased linearly with blood accumulation. Further, the change in oxygenation was monitored with the modulation of the oxygen level in the gas supply. Finally, tMRO changes were measured during ischemia, induced by the upper arm cuff and the results showed a decrease and a recovery of tMRO with cuff inflation and deflation, respectively. This simple diffuse optical metabolic spectroscopic system can easily be applied in medical environments by providing a simple and convenient solution for measuring tMRO.