AI Article Synopsis
- Mammalian epidermal stem cells help keep the skin healthy and regenerate it throughout adult life, but effective long-term culture systems have been lacking.
- A new epidermal organoid culture system allows for the stable expansion of these stem cells without the need for feeder cells or serum, mimicking the natural structure of mouse skin.
- This innovative system utilizes a specific combination of signaling factors to maintain the organization of the skin cells and offers a valuable method for studying skin health and disease in the lab.
Article Abstract
Mammalian epidermal stem cells maintain homeostasis of the skin epidermis and contribute to its regeneration throughout adult life. While 2D mouse epidermal stem cell cultures have been established decades ago, a long-term, feeder cell- and serum-free culture system recapitulating murine epidermal architecture has not been available. Here we describe an epidermal organoid culture system that allows long-term, genetically stable expansion of adult epidermal stem cells. Our epidermal expansion media combines atypically high calcium concentrations, activation of cAMP, FGF, and R-spondin signaling with inhibition of bone morphogenetic protein (BMP) signaling. Organoids are established robustly from adult mouse skin and expand over at least 6 mo, while maintaining the basal-apical organization of the mouse interfollicular epidermis. The system represents a powerful tool to study epidermal homeostasis and disease in vitro.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6642409 | PMC |
| http://dx.doi.org/10.1073/pnas.1715272116 | DOI Listing |
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