Aims: The diversity and the geographical distribution of swine papillomaviruses (PVs) are virtually unknown. The occurrence and the diversity of swine PV were therefore investigated in pig slurry collected in Italy, to contribute towards filling this gap in knowledge.
Methods And Results: Twenty-two slurry samples underwent analysis by nested PCR and DNA sequencing using published and newly designed specific primer pairs for Sus scrofa papillomavirus (SsPV) type 1 and 2 (SsPV1 and 2), along with degenerate PV-specific primers targeting the major coat protein L1 and the helicase protein E1. Overall, three samples (13·6%) were positive for SsPV1 by specific primers, and nucleotide (nt) sequences showed 99-100% nt identity with SsPV1 variant a (EF395818), while SsPV2 was not found in any sample. Using generic primers, eight samples (36·4%) were tested positive for human papillomavirus (HPV), and were characterized as follows: β1-HPV8, β1-HPV14, β1-HPV206, β2-HPV113, β2-HPV120 and γ1-HPV173. Moreover, one unclassified γ-type was detected.
Conclusions: Both swine and human PVs were detected in pig slurry in this study. The unexpected presence of HPV in pig waste could be explained as the result of an improper use of the sewage collection pits and/or with improper procedures of the operators.
Significance And Impact Of The Study: This study reports the first detection of SsPV1 in Italy, along with the first detection of HPVs in pig slurry samples in Italy, and expands our knowledge about PV diversity and geographic distribution.
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http://dx.doi.org/10.1111/jam.14363 | DOI Listing |
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January 2025
Sichuan Academy of Eco-Environmental Sciences, Chengdu, 610041, China.
The widespread application of swine-farming wastewater to soil and water is increasingly contributing to heavy metal contamination, posing significant environmental risks. This study investigated the concentrations of eight heavy metals in swine-farming wastewater following different treatment processes, and assessed their ecological risks in Sichuan Province, China. The findings revealed that zinc, copper and nickel exhibited the highest concentrations, potentially causing heavy or strong contamination levels and leading to heavy or slight ecological risks.
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Estación Experimental de Aula Dei, EEAD - CSIC, Ave. Montañana 1005, 50059 Zaragoza, Spain.
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January 2025
Agroécologie, French National Institute for Agriculture, Food, and Environment (INRAE), Institut Agro, Univ. Bourgogne, Univ. Bourgogne Franche-Comté, Dijon, France.
Anaerobic digestion represents an opportunity for converting organic waste (OW) into valuable products: renewable energy (biogas) and a fertilizer (digestate). However, the long-term effects of digestates on soil biota, especially microorganisms, need to be better documented to understand the impact of digestate on soil ecosystem functioning and resilience. This study assessed the cumulative effect of repeated pig slurry digestate applications on soil microbial communities over a decade, using an in-situ approach to compare digested feedstock with undigested feedstock and other fertilization treatments.
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March 2025
LNEG, National Laboratory of Energy and Geology I.P., Bioenergy and Biorefineries Unit, Estrada do Paço do Lumiar 22, Lisbon 1649-038, Portugal; GreenCoLab, Green Ocean Technologies and Products Collaborative Laboratory, University of Algarve, Campus de Gambelas, Faro 8005-139, Portugal.
Pig farming generates highly polluted wastewater that requires effective treatment to minimize environmental damage. Microalgae can recover nutrients from piggery wastewater (PWW), but excessive nutrient and turbidity levels inhibit their growth. Solar photo-Fenton (PF) offer a sustainable and cost-effective pretreatment to allow microalgal growth for further PWW treatment.
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Centro de Investigación e Innovación Agroalimentaria y Agroambiental (CIAGRO-UMH), Universidad Miguel Hernández, Carretera de Beniel Km 3,2, Orihuela, Alicante 03312, Spain.
Olive mill wastes (OMW) management by composting allows to obtain valuable fertilizing products, but also implies significant fluxes of greenhouse gases (GHG). For a proper OMW composting, high C- and N co-substrates are necessary, but little is known concerning their effect on GHG emissions in OMW-industrial scale composting. In this study, different co-composting agents (cattle manure (CM), poultry manure (PM), sheep manure (SM) and pig slurry solid fraction (PSSF) as N sources and olive leaves (OLW) and urban pruning residues (UPR) as bulking agents and C sources) were used for OMW composting at industrial scale.
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