Bioactive constituents from seeds of Persoon (Annonaceae).

Column chromatography led to the isolation and full characterization of -cerotoyltryptamine () previously described in a mixture of 5 compounds, asimicin () and -19-Carbomethoxykauran-17-oic acid () isolated from this species for first time alongside stigmasterol glycoside () and lacceroic acid (). The structures of the compounds were established by extensive EIMS, HRESIMS, 1 D and 2 D NMR studies. Compound and the extract AS were more potent inhibitors of ROS with IC values of 2.7 ± 0.1 µg/mL and 8.7 ± 10.2 µg/mL respectively than Ibuprofen (11.2 ± 1.9 µg/mL) as a standard anti-inflammatory drug. Compound showed high inhibition on nitric oxide (IC = 3.9 ± 0.2 µg/mL), almost 6 times more active than the standard compound L-NMMA (IC = 24.2 ± 0.8 µg/mL) used. Compounds showed relatively low toxicity on NIH-3T3 fibroblast cells compared to standard. The results indicate that compounds and are potent anti-inflammatory drug candidates.