Paenibacillus nuruki sp. nov., isolated from Nuruk, a Korean fermentation starter.

A Gram-stain-positive, rod-shaped, non-endospore-forming motile by means of peritrichous flagella, facultatively anaerobic bacterium designated TI45-13ar was isolated from Nuruk, a Korean traditional Makgeolli fermentation starter. It grew at 4-35°C (optimum, 28-30°C), pH 5.0-9.0 (optimum, pH 7.0) and NaCl concentrations up to 5% (w/v). Phylogenetic trees generated using 16S rRNA gene sequences revealed that strain TI45-13ar belonged to the genus Paenibacillus and showed the highest sequence similarities with Paenibacillus kyungheensis DCY88 (98.5%), Paenibacillus hordei RH-N24 (98.4%) and Paenibacillus nicotianae YIM h-19 (98.1%). The major fatty acid was anteiso-C. The DNA G+C content was 39.0 mol%, and MK-7 was the predominant isoprenoid quinone. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unidentified glycolipids, and one unidentified aminoglycolipid. The cell-wall peptidoglycan contained meso-diaminopimelic acid. On the basis of polyphasic taxonomy study, it was suggested that strain TI45-13ar represents a novel species within the genus Paenibacillus for which the name Paenibacillus nuruki sp. nov. is proposed. The type strain was TI45-13ar (= KACC 18728 = NBRC 112013).