AI Article Synopsis
- This study focuses on using live imaging techniques to observe the movement of fluorescent proteins and vesicles in isolated axons.
- The researchers specifically examine the behavior of ESCRT proteins in axons of primary hippocampal neurons.
- The chapter provides a detailed methodology for preparing microfluidic chambers, isolating neurons, and transducing them with lentiviruses for dynamic analysis.
Article Abstract
Live imaging of microfluidically isolated axons permits study of the dynamic behavior of fluorescently tagged proteins and vesicles in these neuronal processes. We use this technique to study the motility and transport of ESCRT proteins in axons of primary hippocampal neurons. This chapter details the preparation of microfluidic chambers, as well as the seeding, fluidic isolation, and lentiviral transduction of hippocampal neurons in these chambers, optimized for the study of ESCRT protein dynamics.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8882341 | PMC |
| http://dx.doi.org/10.1007/978-1-4939-9492-2_9 | DOI Listing |
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