Severity: Warning
Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
Filename: helpers/my_audit_helper.php
Line Number: 144
Backtrace:
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 144
Function: file_get_contents
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 212
Function: simplexml_load_file_from_url
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3106
Function: getPubMedXML
File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global
File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword
File: /var/www/html/index.php
Line: 316
Function: require_once
A Gram-stain-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, MRS2, was isolated from an intestine of Nile tilapia () collected from the Republic of Korea. Strain MRS2 grew optimally at 30 °C and in the presence of 0-2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain MRS2 clustered with the type strains of species. It exhibited the highest 16S rRNA gene sequence similarity (98.5 %) to the type strain of and sequence similarities of 97.4-97.6 % to the type strains of two other species. Strain MRS2 contained MK-6 as the predominant ubiquinone and summed feature 3 (Cω7 and/or Cω6), iso-C 3-OH and iso-C as the major fatty acids. The major polar lipids of strain MRS2 were phosphatidylethanolamine, one unidentified lipid and one unidentified aminolipid. The DNA G+C contents of strain MRS2 were 32.2 mol% or 30.65 mol%. Strain MRS2 exhibited DNA-DNA relatedness values of 12-20 % to the type strains of , and . The average nucleotide identity values between strain MRS2 and five strains of and were 84.8-91.0 %. The phylogenetic, genetic and differential phenotypic properties indicated that strain MRS2 is separated from species. On the basis of the data presented here, strain MRS2 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MRS2 (=KCTC 62904=NBRC 113550).
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Source |
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http://dx.doi.org/10.1099/ijsem.0.003558 | DOI Listing |
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