AI Article Synopsis
- Mammalian sperm need a process called capacitation to fertilize an egg, which involves changes in the sperm's membrane.
- While mouse studies have shown that hyperpolarization of the sperm plasma membrane is important for this process, reliable methods to measure this in human sperm have been lacking.
- This report introduces a new fluorimetric population assay to measure human sperm membrane potential, alongside validation through flow cytometry, laying the groundwork for further research into its role in human sperm capacitation.
Article Abstract
Mammalian sperm must undergo a complex process called capacitation in order to fertilize the egg. During this process, hyperpolarization of the sperm plasma membrane has been mostly studied in mouse, and associated to its importance in the preparation to undergo the acrosome reaction (AR). However, despite the increasing evidence of membrane hyperpolarization in human sperm capacitation, no reliable techniques have been set up for its determination. In this report we describe human sperm membrane potential () measurements by a fluorimetric population assay, establishing optimal conditions for determination. In addition, we have conducted parallel measurements of the same human sperm samples by flow cytometry and population fluorimetry, before and after capacitation, to conclusively address their reliability. This integrative analysis sets the basis for the study of in human sperm allowing future work aiming to understand its role in human sperm capacitation.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6579818 | PMC |
| http://dx.doi.org/10.3389/fcell.2019.00101 | DOI Listing |
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