AI Article Synopsis
- Fusarium head blight (FHB) is a major wheat disease that significantly affects the economy, with a key genetic marker identified for resistance.
- Researchers developed a Kompetitive Allele Specific PCR (KASP) marker, named PFT_KASP, which effectively detects FHB resistance in various wheat breeding lines.
- The study revealed discrepancies between the new KASP marker and existing markers, highlighting PFT_KASP as a precise tool for breeding resistant wheat varieties.
Article Abstract
Fusarium head blight (FHB) is a devastating wheat disease with a significant economic impact. is the most important large effect and stable QTL for FHB resistance. A pore-forming toxin-like () gene was recently identified as an underlying gene for resistance. In this study, we developed and validated a -based Kompetitive allele specific PCR (KASP) marker for . The KASP marker, PFT_KASP, was used to screen 298 diverse wheat breeding lines and cultivars. The KASP clustering results were compared with gel-based gene specific markers and the widely used linked STS marker, UMN10. Eight disagreements were found between PFT_KASP and UMN10 assays among the tested lines. Based on the genotyping and sequencing of genes in the region, these genotypes were found to be common with a previously characterized susceptible haplotype. Therefore, our results indicate that PFT_KASP is a perfect diagnostic marker for and would be a valuable tool for introgression and pyramiding of FHB resistance in wheat cultivars.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6586189 | PMC |
| http://dx.doi.org/10.5423/PPJ.OA.01.2019.0018 | DOI Listing |
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