AI Article Synopsis
- Despite advancements in organs-on-a-chip technology, integrating real-time sensing for biomarkers in 3D tissues has been limited.
- The study introduces a custom platform for stimulating muscle cells in a fluid environment, connected to a sensitive electrochemical sensing system for monitoring interleukin 6 and tumor necrosis factor alpha.
- This innovative setup allows for precise, cost-effective measurements of biomarker release from 3D muscle microtissues during different stimulations, supporting metabolic research.
Article Abstract
Despite the increasing number of organs-on-a-chip that have been developed in the past decade, limited efforts have been made to integrate a sensing system for in situ continual measurements of biomarkers from three-dimensional (3D) tissues. Here, we present a custom-made integrated platform for muscle cell stimulation under fluidic conditions connected with a multiplexed high-sensitivity electrochemical sensing system for in situ monitoring. To demonstrate this, we use our system to measure the release levels and release time of interleukin 6 and tumor necrosis factor alpha in vitro by 3D muscle microtissue under electrical and biological stimulations. Our experimental design has enabled us to perform multiple time point measurements using functionalized screen-printed gold electrodes with sensitivity in the ng mL range. This affordable setup is uniquely suited for monitoring factors released by 3D single cell types upon external stimulation for metabolic studies.
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| http://dx.doi.org/10.1039/c9lc00285e | DOI Listing |
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