Different vasodilator mechanisms in intermediate- and small-sized arteries from the hindlimb vasculature of the toad .

Am J Physiol Regul Integr Comp Physiol

Deakin University, School of Life and Environmental Sciences, Waurn Ponds Campus, Geelong, Victoria, Australia.

Published: September 2019

In this study, myography was used to determine the effect of arterial size on nitric oxide (NO) vasodilatory mechanisms in the hindlimb vasculature of the toad . Immunohistochemical analysis showed NO synthase (NOS) 1 immunoreactivity in perivascular nitrergic nerves in the iliac and sciatic arteries. Furthermore, NOS3 immunoreactivity was observed in the vascular smooth muscle of the sciatic artery, but not the endothelium. Acetylcholine (ACh) was used to facilitate intracellular Ca signaling to activate vasodilatory pathways in the arteries. In the iliac artery, ACh-mediated vasodilation was abolished by blockade of the soluble guanylate cyclase pathway with the soluble guanylate cyclase inhibitor ODQ (1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one, 10 M) and blockade of the prostaglandin signaling pathway with indomethacin (10 M). Furthermore, disruption of the endothelium had no effect on the ACh-mediated vasodilation in the iliac artery, and generic inhibition of NOS with -nitro-l-arginine (3 × 10 M) significantly inhibited the vasodilation, indicating NO signaling. In contrast to the iliac artery, ACh-mediated vasodilation of the sciatic artery had a significant endothelium-dependent component. Interestingly, the vasodilation was not significantly affected by -nitro-l-arginine, but it was significantly inhibited by the specific NOS1 inhibitor -(1-imino-3-butenyl)-l-ornithine (vinyl-l-NIO, 10 M). ODQ mostly inhibited the ACh-mediated vasodilation. In addition, indomethacin also significantly inhibited the ACh-mediated vasodilation, indicating a role for prostaglandins in the sciatic artery. This study found that the mechanisms of vasodilation in the hindlimb vasculature of vary with vessel size and that the endothelium is involved in vasodilation in the smaller sciatic artery.

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http://dx.doi.org/10.1152/ajpregu.00319.2018DOI Listing

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