ELISAs and rapid diagnostic tests (RDTs) are widely used for diagnosing dengue virus (DENV) infection. Using 138 single blood samples, we compared the ability to detect non-structural (NS)-1 antigen and anti-DENV IgM/IgG antibodies among (1) DENV Detect NS1 ELISA, DENV Detect IgM capture ELISA and DENV Detect IgG ELISA (InBios International, Inc.); (2) Anti-Dengue virus IgM Human ELISA and Anti-Dengue virus IgG Human ELISA (Abcam); (3) Dengue virus NS1 ELISA, Anti-Dengue virus ELISA (IgM) and Anti-Dengue virus ELISA (IgG) (Euroimmun); (4) Asan Easy Test Dengue NS1 Ag 100 and Asan Easy Test Dengue IgG/IgM (Asan Pharm); (5) SD BIOLINE Dengue Duo (Standard Diagnostics); and (6) Ichroma Dengue NS1 and Ichroma Dengue IgG/IgM (Boditech Med). For NS1 antigen detection, InBios and Euroimmun showed higher sensitivities (100%) than the RDTs (42.9-64.3%). All tests demonstrated variable sensitivities for IgM (38.1-90.5%) and IgG (65.7-100.0%). InBios and Boditech Med demonstrated higher sensitivity (95.6% and 88.2%, respectively) than the other tests for combined NS1 antigen and IgM antibody. Five NS1 antigen tests had good agreement (92.8-98.6%) without showing positivity for chikungunya. However, all IgG tests demonstrated potential false-positivity with variable ranges. Clinical laboratories should note performance variations across tests and potential cross-reactivity.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6660329 | PMC |
| http://dx.doi.org/10.3343/alm.2019.39.6.566 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Investigation of risk factors for household-based dengue virus infection in Borobudur Subdistrict, Magelang, Indonesia.
Germs
September 2024
MD, MPH, PhD, Department of Public Health, Faculty of Medicine, Universitas Islam Indonesia, Kaliurang Street KM 14.5 Yogyakarta 55584, Indonesia.
Introduction: Dengue infection poses a serious threat to global public health, including Indonesia. The rapid spread and significant economic impact are crucial concerns for control efforts. Investigating risk factors of dengue virus infection is necessary to formulate effective strategies, particularly at the household level.
View Article and Find Full Text PDFDifferential Transcriptional Landscape of Vero Cells During Dengue Virus 2 Infection in the Presence of Sinococuline.
Microorganisms
December 2024
Translational Health, Molecular Medicine Division, International Centre for Genetic Engineering & Biotechnology, New Delhi 110067, India.
Dengue virus (DENV) is transmitted by genus mosquitoes and is responsible for dengue fever (DF) and other severe diseases, posing a significant challenge to the global health system. Currently, anti-dengue pharmacological treatment options are not available. Earlier, we demonstrated that Sinococuline has potent anti-dengue activity and prevents virus infection.
View Article and Find Full Text PDFClinical and Laboratory Features and Treatment Outcomes of Dengue Fever in Pediatric Cases.
Cureus
December 2024
Department of Pharmacology and Therapeutics, National Defence University of Malaysia, Kuala Lumpur, MYS.
Background Globally, dengue fever (DF) is the leading cause of arthropod-borne viral illness, which considerably contributes to an atrocious death rate. The disease is now endemic in some parts of the world, including Bangladesh. The disorder exhibits a wide range of clinical and laboratory features in children.
View Article and Find Full Text PDFSignificance of diagnostic and therapeutic potential of serum endothelial and inflammatory biomarkers in defining disease severity of dengue infected patients.
Med Microbiol Immunol
December 2024
Department of Biochemistry and Medical Biotechnology, Calcutta School of Tropical Medicine, 108, C. R. Avenue, Kolkata, 700073, West Bengal, India.
Dengue virus (DENV) mediated disease severity leads to fatality among infected patients. Immune sentinels recognize DENV thereby secreting inflammatory mediators, endothelial biomarkers and anticoagulation factors. Absence of any diagnostic biomarkers for early identification of severe dengue (SD) patients has hindered disease management.
View Article and Find Full Text PDFProspects of in the treatment of human viral infections: A comprehensive and a systematic review.
Heliyon
November 2024
Atta-ur-Rehman School of Applied Biosciences, National University of Sciences and Technology H-12 Islamabad, Islamabad Capital Territory, Pakistan.
Background: Viruses cause various human diseases, some of which become pandemic outbreaks lack effective treatments and vaccines. This study gathered data on Carica papaya's antiviral effects against various human viruses, highlighting its potential as a natural treatment. Through , , and clinical study analysis, we illustrated the potential of Carica papaya in combating viral infections and its potential role in cure of common viral diseases.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!