Vitamin D Receptor Signaling Regulates Craniofacial Cartilage Development in Zebrafish.

Vitamin D plays essential roles in supporting the skeletal system. The active form of vitamin D functions through the vitamin D receptor (VDR). A hereditary vitamin-D-resistant rickets with facial dysmorphism has been reported, but the involvement of VDR signaling during early stages of craniofacial development remains to be elucidated. The present study investigated whether VDR signaling is implicated in zebrafish craniofacial cartilage development using a morpholino-based knockdown approach. Two paralogous genes, and , have been found in zebrafish embryos. Loss-of- has no discernible effect on cartilage elements, whereas loss-of- causes reduction and malformation of craniofacial cartilages. Disrupting both and leads to more severe defects or complete loss of cartilage. Notably, knockdown of results in elevated expression of (), a bone morphogenetic protein (BMP) antagonist, in the adjacent pharyngeal endoderm. Taken together, these findings strongly indicate that VDR signaling is required for early craniofacial cartilage development in zebrafish.