AI Article Synopsis

  • The basic helix-loop-helix (bHLH) family is a major group of transcription factors that helps regulate growth and development in plants, but research on this family in safflower is limited.
  • In this study, 41 bHLH genes were identified in safflower, classified into 23 subgroups, and 10 conserved protein motifs were found through phylogenetic analysis.
  • The research also focused on bHLH genes related to flavonoid biosynthesis using methods like gene expression analysis and protein interaction prediction, contributing to a better understanding of safflower's biochemical and physiological processes.

Article Abstract

The basic helix-loop-helix (bHLH) family is the second largest superfamily of transcription factors that belongs to all three eukaryotic kingdoms. The key function of this superfamily is the regulation of growth and developmental mechanisms in plants. However, the bHLH gene family in has not yet been studied. Here, we identified 41 bHLH genes in that were classified into 23 subgroups. Further, we conducted a phylogenetic analysis and identified 10 conserved protein motifs found in the safflower bHLH family. We comprehensively analyzed a group of bHLH genes that could be associated with flavonoid biosynthesis in safflower by gene expression analysis, gene ontology annotation, protein interaction network prediction, subcellular localization of the candidate CtbHLH40 gene, and real-time quantitative expression analysis. This study provides genome-wide identification of the genes related to biochemical and physiological processes in safflower.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6627405PMC
http://dx.doi.org/10.3390/ijms20123044DOI Listing

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