AI Article Synopsis
- - A study was conducted on 75 clinical isolates of Acinetobacter baumannii from five centers in India to analyze the presence of β-lactamase genes and to compare strains with international clones.
- - The research confirmed that all isolates had certain genes associated with antibiotic resistance, notably Class D and B carbapenemases, with ST-848 being a prominent sequence type indicating a new lineage of concern in spreading resistance.
- - Findings revealed that most isolates clustered into clonal complex 208, linked to an international lineage, highlighting a potential public health issue with increasing carbapenem resistance in the region.
Article Abstract
Background & Objectives: Acinetobacter baumannii is an opportunistic pathogen responsible for causing nosocomial infections. A. baumannii develops resistance to various antimicrobial agents including carbapenems, thereby complicating the treatment. This study was performed to characterize the isolates for the presence of various β-lactamases encoding genes and to type the isolates to compare our clones with the existing international clones across five centres in India.
Methods: A total 75 non-repetitive clinical isolates of A. baumannii from five different centres were included in this study. All the isolates were confirmed as A. baumannii by bl aPCR. Multiplex PCR was performed to identify the presence of extended spectrum β-lactamases (ESBL) and carbapenemases. Multilocus sequence typing was performed to find the sequence type (ST) of the isolates. e-BURST analysis was done to assign each ST into respective clonal complex.
Results: blawas present in all the 75 isolates. The predominant Class D carbapenemase was blafollowed by Class B carbapenemase, bla. Class A carbapenemase was not observed. blawas the predominant extended spectrum β-lactamase. ST-848, ST-451 and ST-195 were the most common STs. Eight-novel STs were identified. e-BURST analysis showed that the 75 A. baumannii isolates were clustered into seven clonal complexes and four singletons, of which, clonal complex 208 was the largest.
Interpretation & Conclusions: Most of the isolates were grouped under clonal complex 208 which belongs to the international clonal lineage 2. High occurrence of ST-848 carrying blagene suggested that ST-848 could be an emerging lineage spreading carbapenem resistance in India.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6563728 | PMC |
| http://dx.doi.org/10.4103/ijmr.IJMR_2085_17 | DOI Listing |
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