AI Article Synopsis
- Myostatin (MSTN) is being explored as a potential treatment target for muscle degeneration and as a biomarker for targeting engagement.
- Researchers developed a sensitive 2D-LC-MS/MS assay to quantify MSTN levels in various animal species, with effective sample preparation involving protein denaturation and peptide enrichment.
- The assay demonstrated strong validation results with a lower limit of quantification at 2.5 ng/ml in rat and monkey serum, showing high precision and accuracy, and was successfully used to measure MSTN in mouse, rat, monkey, and human serum, revealing increased MSTN levels in rat and monkey serum after MSTN inhibitor administration.
Article Abstract
Myostatin (MSTN) is an attractive therapeutic target for the treatment of muscle degeneration-related diseases and is being evaluated as a target engagement biomarker. A sensitive 2D-LC-MS/MS assay was developed to quantify MSTN in different animal species. Sample preparation involved SDS denaturation of serum proteins followed by tryptic digestion and peptide enrichment by SPE. The assay was validated with LLOQ of 2.5 ng/ml in rat and monkey serum. The precision was within 13.7%, and the bias was within ±12.6% for all quality control samples in authentic matrices. This new assay was successfully applied to measure MSTN in mouse, rat, monkey and human serum. The total MSTN in rat and monkey serum was elevated following administration of an MSTN inhibitor.
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| http://dx.doi.org/10.4155/bio-2018-0311 | DOI Listing |
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