Antimicrobial Susceptibility and Molecular Characterization of Clinical Strains of in Western Saudi Arabia.

has emerged as a problematic multidrug-resistant (MDR) pathogen worldwide. The aim of this study was to characterize clinical isolates of from Jeddah, a coastal city in the western region of Saudi Arabia that has an ∼50% expatriate population. In this study, 135 nonrepetitive clinical isolates of were identified through MALDI-TOF and susceptibility was determined with VITEK-2 systems and broth microdilution method. Genotypic characterization of the isolates was performed by using M13 primer typing and polymerase chain reaction screening was performed for carbapenemase genes, insertion sequences, metallo-β-lactamases, and cephalosporinase genes. The isolates were recovered from heterogeneous clinical specimens, and the majority of the cases of infection were acquired in the hospital and predominantly involved patients who were older than 50 years. Total, 58.5% of the isolates were MDR, and 55.6% isolates were resistant to carbapenem antibiotics. Approximately half of the isolates were resistant to ceftazidime, and cefepime among the β-lactam antibiotics and ciprofloxacin from the quinolone group. The gene and IS upstream of were detected in 92% of the carbapenem-resistant isolates, while all carbapenem-resistant isolates were found to carry and -type cephalosporinase gene. The gene was detected in 84% isolates, and two isolates carried the gene. Data demonstrate the coexistence of multiple carbapenem resistance determinants in from the western region of Saudi Arabia.