Reactive oxygen species altering the metabolite profile of the marine-derived fungus F31-1.

To investigate the influence of reactive oxygen species (ROS) on the secondary metabolites of the marine-derived fungus F31-1, hydrogen peroxide (HO) was added to the GPY culture medium. The HPLC chromatogram of the EtOAc extract of the culture broth was distinct from that of the HO free GPY medium. Further study of the metabolites in the GPY medium with HO resulted in the discovery of eight known compounds. Among them, (22)-5α, 8α-epidioxyergosta-6, 22-dien-3-ol () and ergosta-4,6,8(14),22-tetraene-3-one () were present in the highest concentration, while ergosterol and diketopiperazines are abundant in the HO free medium. Additionally, a new compound, dichocetide D () containing a chlorine element and a known ergosterol () were isolated from the HO free medium. (22)-5α, 8α-epidioxyergosta-6, 22-dien-3-ol () exhibited moderate cytotoxic activity against human prostate cancer cell line LNCaP-C4-2B.