The Association of Official Analytical Chemists (AOAC) and the Canadian Health Protection Branch (CHPB) methods for detection of Salmonella spp. were compared for recovery of Salmonella javiana from experimentally contaminated mozzarella cheese. Ten trials were conducted with two levels of random surface contamination of cheese. Five samples per level of contamination were randomly chosen and analyzed by each method. The results showed that there was no significant difference between the two methods ( < 0.05). When S. javiana was directly added to shredded cheese and analyzed immediately, the recovery was identical with both methods; 7 of 10 samples were positive by each method. However, when the directly contaminated cheese was stored for 7 days and analyzed by both methods, the results differed. The AOAC method showed only 3 positive samples, whereas the CHPB yielded 6, compared to 7 positive samples before storage by both methods. During preenrichment the pH decreased to a greater extent with the AOAC method (7.4 to 4.4) than with the CHPB method (7.6 to 5.7) in 24 h; this pH decrease resulted in 1 log higher number of S. javiana counts in the latter (10 CFU/ml and 10 ml).
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.4315/0362-028X-60.12.1493 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Efficiency of Bacteriophage-Based Detection Methods for Non-Typhoidal in Foods: A Systematic Review.
Viruses
November 2024
Centre for Epidemiology and Planetary Health, School of Veterinary Medicine, Scotland's Rural College, Inverness IV2 5NA, UK.
Food contamination with non-typhoidal (NTS) presents a significant public health risk, underscoring the critical need for rigorous food safety measures throughout the production, distribution, preparation, and consumption stages. Conventional diagnostic strategies are time-consuming and labor-intensive and are thus sub-optimal for throughput NTS detection. Bacteriophages (phages) are highly specialized bacterial viruses and exhibit extreme specificity for their hosts.
View Article and Find Full Text PDFAntimicrobial Susceptibility Profiles of spp. Isolates from Clinical Cases of Waterfowl in Hungary Between 2022 and 2023.
Microorganisms
November 2024
Department of Pharmacology and Toxicology, University of Veterinary Medicine, István utca 2, 1078 Budapest, Hungary.
The global spread of antimicrobial resistance is one of the most significant challenges of the 21st century. The waterfowl sector is an economically decisive part of the poultry industry, yet it remains under-researched, and its antibiotic usage is less monitored. Our study aimed to determine the antimicrobial susceptibility of avian pathogenic strains, which are still prevalent in ducks and geese, against antibiotics critical for both animal and human health, and to compare these findings with human resistance data.
View Article and Find Full Text PDFYear-Long Prevalence and Antibiotic Resistance Profiles of Serogroups Isolated from a Wisconsin Dairy Farm.
Pathogens
November 2024
Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA.
infections can significantly impact the health and productivity of dairy cattle. Asymptomatic carriage of can make it difficult to identify and monitor this pathogen across a herd. Therefore, a more focused census on dairy farms is needed to better understand the dynamics of asymptomatic carriage.
View Article and Find Full Text PDFResearch Note: Evaluation of selected chlorhexidine salts/product and/or cetylpyridinium chloride as potential pre-slaughter crop disinfection agents using an in vitro assay simulating crop contents.
Poult Sci
December 2024
Department of Poultry Science, University of Arkansas Division of Agriculture, Fayetteville 72701, USA.
Salmonellae remain a prominent issue for poultry products regarding food safety. A predominant source of broiler carcass contamination at processing is from crop rupturing and subsequent contamination of the carcass during evisceration. Chlorhexidine (CHX) salts are commonly used in dental products for their antimicrobial capabilities.
View Article and Find Full Text PDFGenomic analysis, culturing optimization, and characterization of bacteriophage OSYSP, previously studied as effective pathogen control on fresh produce.
Front Microbiol
December 2024
Department of Food Science and Technology, The Ohio State University, Columbus, OH, United States.
Advances in bacteriophage genome sequencing and regulatory approvals of some bacteriophages in various applications have renewed interest in these antibacterial viruses as a potential solution to persistent food safety challenges. Here, we analyzed in depth the genome of the previously studied bacteriophage OSYSP (phage OSYSP), revealed its application-related characteristics, and optimized its enumeration techniques for facilitating industrial implementation. We previously sequenced phage OSYSP genome completely by combining results from Illumina Miseq and Ion Torrent sequencing platforms and completing the remaining sequence gaps using PCR.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!