Yellow fever virus (YFV) is responsible for devastating outbreaks of Yellow fever (YF) in humans and is associated with high mortality rates. Recent large epidemics and epizootics and exponential increases in the numbers of YF cases in humans and non-human primates highlight the increasing threat YFV poses, despite the availability of an effective YFV vaccine. YFV is the first human virus discovered, but the structures of several of the viral proteins remain poorly understood. Here we report the structure of the full-length NS5 protein, a key enzyme for the replication of flaviviruses that contains both a methyltransferase domain and an RNA dependent RNA polymerase domain, at 3.1 Å resolution. The viral polymerase adopts right-hand fold, demonstrating the similarities of the Yellow fever, Dengue and Zika polymerases. Together this data suggests NS5 as a prime and ideal target for the design of pan-flavivirus inhibitors.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.antiviral.2019.104536 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Reaching Forest Workers with Yellow Fever Vaccine Through Engagement of the Private Sector in Central African Republic.
Vaccines (Basel)
December 2024
World Health Organization, 20 Avenue Appia, 1211 Geneva, Switzerland.
: Yellow fever (YF) outbreaks continue to affect populations that are not reached by routine immunization services, such as workers at a high risk of occupational exposure to YF. In the Central African Republic (CAR), YF cases were detected in districts characterized by the presence of workers in forest areas. We developed an innovative approach based on a local partnership with private companies of the extractive industry to administer YF vaccine to workers in remote areas during the response to an outbreak.
View Article and Find Full Text PDFDifferential Neutralization Profiles of 17DD Vaccinated Population to 17D-204 and 17DD Vaccine Strains.
Vaccines (Basel)
November 2024
Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555-0609, USA.
: Yellow fever virus (YFV) (, ) is the etiologic agent of yellow fever (YF), a vector-borne disease with significant morbidity and mortality across the tropics and neotropics, despite having a highly efficacious and safe vaccine (17D). Vaccination provides lifelong protection from YF disease mediated by humoral immunity. There are several versions of the original 17D vaccine: 17D-204 (marketed in the USA as YF-VAX, in France as Stamaril, and in China as Tiantan-V), 17D-213 (Russian Federation), and 17DD (by FIOCRUZ in Brazil).
View Article and Find Full Text PDFPreparation of -Myrcene-Chitosan Nanoparticles and Their Uptake and Toxicity in Larvae.
Insects
December 2024
Departamento de Entomologia, Universidade Federal de Viçosa (UFV), Viçosa 36570-900, MG, Brazil.
Mosquito control still relies heavily on synthetic molecules, which can lead to the selection of resistant populations and undesirable environmental problems. This study described the preparation of a nanoparticle of the plant-derived molecule, -myrcene, with chitosan, and the assessment of its toxicity against larvae of the yellow fever mosquito, . By producing fluorescent chitosan nanoparticles, we were able to observe their distribution in the digestive tract of larvae of .
View Article and Find Full Text PDFRole of extracellular vesicles in the pathogenesis of mosquito-borne flaviviruses that impact public health.
J Biomed Sci
January 2025
Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México (UNAM), 04510, Mexico City, Mexico.
Mosquito-borne flaviviruses represent a public health challenge due to the high-rate endemic infections, severe clinical outcomes, and the potential risk of emerging global outbreaks. Flavivirus disease pathogenesis converges on cellular factors from vectors and hosts, and their interactions are still unclear. Exosomes and microparticles are extracellular vesicles released from cells that mediate the intercellular communication necessary for maintaining homeostasis; however, they have been shown to be involved in disease establishment and progression.
View Article and Find Full Text PDFDengue and Zika virus NS4B proteins differ in topology and in determinants of ER membrane protein complex dependency.
J Virol
December 2024
1Division of Gastroenterology, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Flaviviruses utilize the cellular endoplasmic reticulum (ER) for all aspects of their lifecycle. Genome replication and other viral activities take place in structures called replication organelles (ROs), which are invaginations induced in the ER membrane. Among the required elements for RO formation is the biogenesis of viral nonstructural proteins NS4A and NS4B.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!