CoO nanoparticles supported mesoporous carbon framework interface for glucose biosensing.

The present work reports the preparation of advanced functional nanostructures based on cobalt oxide supported mesoporous carbon framework (CoO@MCF) for electrochemical biosensing. CoO@MCF was synthesized by simple hythrothermal & pyrolysis method and further characterized by various microscopic and spectroscopic techniques. The transmission electron microscopic (TEM) images show the lattice fringes of crystalline CoO with interlayer spacing of 0.24 nm. The characteristic 311 plane in X-ray diffraction (XRD) studies further confirmed the presence of crystalline CoO on carbon frameworks. Reflection of prominent A1g peak along with D and G band in raman spectra confirmed the successful fabrication of CoO@MCF nanocomposite. Prepared CoO@MCF manifested great porosity, good biocompatibility and large surface area which allowed effective immobilization of glucose oxidase (GOx) onto its surface using chitosan (Chi) as a binder. Thus, a nanocomposite (CoO@MCF-Chi-GOx) modified glassy carbon electrode (GCE) was fabricated for highly selective detection of glucose using amperometry and cyclic voltammetry. The CoO@MCF-Chi-GOx/GCE electrode exhibited excellent biosensing performance for glucose monitoring with detection limit of (LOD) of 107.70 μM and reproducibility of 4.7% RSD. Moreover, the biosensor holds great promise for its effective implications in point-of-care diagnostics of small biomolecules.