AI Article Synopsis
- Infection with cagA-positive Helicobacter pylori is linked to gastric cancer, highlighting the need for precise diagnosis.
- Researchers developed a quick and sensitive loop-mediated isothermal amplification (LAMP) test to detect the H. pylori cagA gene, outperforming traditional PCR methods.
- The LAMP test showed high sensitivity for detecting H. pylori in both pure culture and fecal samples, suggesting its potential for improving diagnosis and screening, ultimately aiming to reduce gastric cancer cases.
Article Abstract
Infection with cagA-positive Helicobacter pylori is associated with gastric cancer. Molecular techniques are vital for accurate H. pylori diagnosis. We developed a loop-mediated isothermal amplification (LAMP) for detecting the H. pylori cagA gene and evaluated its use for clinical diagnosis. A LAMP primer set was designed to recognize the homologous regions of cagA gene sequences of 6 H. pylori strains. LAMP sensitivity was evaluated with serial dilutions of H. pylori ATCC 43504 and fecal specimens; specificity was evaluated with H. pylori ATCC 49396 and CIP 104086. The LAMP sensitivity for H. pylori specimens was 10 cfu/tube (reaction time, 37 min), which was 10-fold more sensitive than polymerase chain reaction. LAMP was also highly sensitive and rapid for fecal specimens. It detected cagA gene from ATCC 49396 and CIP 104086. The findings suggest LAMP can be used for diagnosing and screening of H. pylori infections to decrease gastric cancer incidence.
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| http://dx.doi.org/10.1016/j.mimet.2019.105653 | DOI Listing |
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