AI Article Synopsis
- Transposon mutagenesis is a technique used to create random mutations in bacterial genomes, allowing researchers to pinpoint where these mutations occur.
- Creating a sequence-defined transposon mutant library helps study gene functions by inserting a single transposon mutation in almost all nonessential genes of the bacteria.
- This chapter outlines how to develop a transposon mutant library in Staphylococcus aureus using a specific transposon called bursa aurealis.
Article Abstract
Transposon mutagenesis is one of the most widely used strategies to generate a large number of random mutations within a bacterial genome and then to precisely identify the mutated sites. The generation of sequence-defined transposon mutant libraries that are composed of a collection of different mutants, each containing a single transposon insertion mutation within nearly all of the nonessential genes within the genome, is a rapid and reliable way to enhance the study of gene function. In this chapter, we describe the process to generate a sequence-defined transposon mutant library in Staphylococcus aureus utilizing the mariner-based bursa aurealis transposon.
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| http://dx.doi.org/10.1007/978-1-4939-9570-7_3 | DOI Listing |
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