AI Article Synopsis
- Supercritical fluid extraction (SFE) and pressurized-fluid extraction were utilized to remove aflatoxin M from beef liver samples, with optimal conditions being explored for pressures, temperatures, and modifiers.
- The addition of an organic modifier was crucial for achieving high recovery rates of aflatoxin M, while the extracts underwent clean-up and analysis through advanced chromatography techniques.
- The SFE method not only achieved comparable results to traditional solvent extraction methods but also reduced the need for large amounts of sample and solvents by using a carbon dioxide solution modified with acetonitrile and methanol.
Article Abstract
Supercritical fluid extraction (SFE) and a pressurized-fluid-extraction process were applied for the removal of aflatoxin M from beef liver samples. Various pressures, temperatures, quantity of supercritical carbon dioxide, and organic modifiers were investigated to optimize the extraction methods. Organic modifier was found to be essential for quantitative recovery of aflatoxin M. Extracts were cleaned up by solid-phase extraction and were analyzed via high-performance liquid chromatography coupled with fluorescence detection of the trifluoroacetic acid derivative. Solvent-modified carbon dioxide SFE achieved recoveries comparable to an AOAC-approved method involving organic solvent extraction. SFE allowed the traditional amounts of sample and organic solvent to be reduced. Also, the supercritical-fluid extraction permitted the use of carbon dioxide modified with acetonitrile: methanol (2:1) to replace methylene chloride as the organic solvent for the extraction step.
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| http://dx.doi.org/10.4315/0362-028X-60.6.698 | DOI Listing |
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