Anti-biofilm activity of hydromethanolic plant extracts against isolates from bovine mastitis.

Bovine mastitis (BM) presents a high incidence, being one of the major causative agents. Antibiotics comprise the most common therapeutic approach, but due to their indiscriminate use, high rates of increasingly resistant bacterial species have been markedly pointed out. Particularly, possesses a pronounced ability to form biofilms, and therefore, are of pivotal interest due to its alarming pathogenicity. The present study investigates the antibacterial properties of methanol: water extracts, alone and in combination with , against isolates from BM. All isolates and reference strain proved to be good biofilm producers after 24 h of bacterial growth. Individually, the studied plant extracts (PE) lead to a considerable biofilm cells reduction, but their combination revealed to be the most effective strategy. When tested in combination, both extracts led to a 3 and 5 log reduction for ATCC 25923 and 1, respectively. Based on these findings, both PE seem to be promissory antimicrobial agents for upcoming use on dairy industry contaminations, BM and even -triggered food poisoning. Further studies are needed to understand which of the compounds present in the extracts are responsible for the observed effects, including their corresponding modes of action.