Aim: The aim of present study is to investigate the effect of fatty acids on the outer membrane vesicles (OMVs) produced by spp.
Background: spp. is the important member of Gut microbiota that employ OMVs production for interact with host. Besides, dietary fatty acids could influence on determination of gut microbiota composition and immune response. In this regard, we evaluated the effect of fatty acids on the growth and OMVs production of and .
Methods: and were grown on BHI broth with and without palmitic and palmitoleic acids as saturated and unsaturated fatty acids, respectively. OMVs were extracted using multiple centrifugation and tris-ethylene diamine tetra acetic acid (EDTA)-Sodium deoxy cholate buffers. Physicochemical properties of OMVs were detected by electron microscopy (SEM), Bradford Coomassie brilliant blue assay and SDS-PAGE. Data were analyzed with One-way ANOVA using SPSS.
Results: The growths of both were significantly increased by palmitic acid. Nevertheless, palmitoleic acid had no significant effect on them. Palmitic acid significantly decreased and increased the production of OMVs at low and high concentration, respectively. However, the production of OMVs was not significantly affected by palmitic acid. Although palmitoleic acid had a significant decreasing effect on the production of OMVs, it significantly increased the production of OMVs at low concentration.
Conclusion: In conclusion we reported that palmitic acid had a stimulatory effect on the growth of and and had a dose dependent effect on the production of OMVs. Also producing of OMVs was affected by palmitoleic acid in a dose dependent manner.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6536021 | PMC |
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