Eukaryotic gene expression is often tightly regulated by interactions between transcription factors (TFs) and their DNA targets. Yeast one-hybrid (Y1H) is one of the most extensively used methods to discover these interactions. We developed a high-throughput meiosis-directed yeast one-hybrid system using the Magic Markers of the synthetic genetic array analysis. The system has a transcription factor-DNA interaction discovery rate twice as high as the conventional diploid-mating approach and a processing time nearly one-tenth of the haploid-transformation method. The system also offers the highest accuracy in identifying TF-DNA interactions that can be authenticated in vivo by chromatin immunoprecipitation. With these unique features, this meiosis-directed Y1H system is particularly suited for constructing novel and comprehensive genome-scale gene regulatory networks for various organisms.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6673709 | PMC |
| http://dx.doi.org/10.1101/gr.245951.118 | DOI Listing |
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