OpEn-Tag-A Customizable Optogenetic Toolbox To Dissect Subcellular Signaling.

ACS Synth Biol

Faculty of Biology and Signalling research Centres BIOSS and CIBSS , University of Freiburg, Freiburg , 79104 , Germany.

Published: July 2019

Subcellular localization of signal molecules is a hallmark in organizing the signaling network. OpEn-Tag is a modular optogenetic endomembrane targeting toolbox that allows alteration of the localization and therefore the activity of signaling processes with the spatiotemporal resolution of optogenetics. OpEn-Tag is a two-component system employing (1) a variety of targeting peptides fused to and thereby dictating the localization of mCherry-labeled cryptochrome 2 binding protein CIBN toward distinct endomembranes and (2) the cytosolic, fluorescence-labeled blue light photoreceptor cryptochrome 2 as a customizable building block that can be fused to proteins of interest. The combination of OpEn-Tag with growth factor stimulation or the use of two membrane anchor sequences allows investigation of multilayered signal transduction processes as demonstrated here for the protein kinase AKT.

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http://dx.doi.org/10.1021/acssynbio.9b00059DOI Listing

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OpEn-Tag-A Customizable Optogenetic Toolbox To Dissect Subcellular Signaling.

ACS Synth Biol

July 2019

Faculty of Biology and Signalling research Centres BIOSS and CIBSS , University of Freiburg, Freiburg , 79104 , Germany.

Subcellular localization of signal molecules is a hallmark in organizing the signaling network. OpEn-Tag is a modular optogenetic endomembrane targeting toolbox that allows alteration of the localization and therefore the activity of signaling processes with the spatiotemporal resolution of optogenetics. OpEn-Tag is a two-component system employing (1) a variety of targeting peptides fused to and thereby dictating the localization of mCherry-labeled cryptochrome 2 binding protein CIBN toward distinct endomembranes and (2) the cytosolic, fluorescence-labeled blue light photoreceptor cryptochrome 2 as a customizable building block that can be fused to proteins of interest.

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