[Study on the mechanisms underlying overwintering of using iTRAQ-based quantitative proteomic analysis].

Objective: To compare the difference of protein expression between the post-overwintering stage and the diapauses preparation stage in , so as to reveal the mechanisms underlying the overwintering diapause of .

Methods: A quantitative proteomic analysis was performed in before and after overwintering diapause by using isobaric tags for relative and absolute quantification (iTRAQ) labeling.

Results: A total of 244 differentially expressed proteins were identified in before and after overwintering diapause, including 126 up-regulated proteins and 118 down-regulated proteins. iTRAQ-based quantitative proteomic analysis revealed that these differentially expressed proteins were linked to function and energy production and conversion, lipid metabolism, remodeling of cytoskeleton, carbohydrate metabolism, protein transport, molecular chaperones, stress tolerance and metabolic enzymes.

Conclusions: This is the first study to identify the overwintering diapause-related proteins in using proteomics tools, which reveals KEGG pathways and GO terms associated with the overwintering diapauses of pallens. Our findings provide additional understandings pertaining to the mechanisms underlying the overwintering diapauses of .