NosL is a dedicated copper chaperone for assembly of the Cu center of nitrous oxide reductase.

Nitrous oxide reductase (NOR) is the terminal enzyme of the denitrification pathway of soil bacteria that reduces the greenhouse gas nitrous oxide (NO) to dinitrogen. In addition to a binuclear Cu site that functions in electron transfer, the active site of NOR features a unique tetranuclear copper cluster bridged by inorganic sulfide, termed Cu. In copper-limited environments, NOR fails to function, resulting in truncation of denitrification and rising levels of NO released by cells to the atmosphere, presenting a major environmental challenge. Here we report studies of from , which is part of the gene cluster, and encodes a putative copper binding protein. A Δ mutant strain had no denitrification phenotype under copper-sufficient conditions but failed to reduce NO under copper-limited conditions. NOR isolated from Δ cells was found to be deficient in copper and to exhibit attenuated activity. UV-visible absorbance spectroscopy revealed that bands due to the Cu center were unaffected, while those corresponding to the Cu center were significantly reduced in intensity. studies of a soluble form of NosL without its predicted membrane anchor showed that it binds one Cu(i) ion per protein with attomolar affinity, but does not bind Cu(ii). Together, the data demonstrate that NosL is a copper-binding protein specifically required for assembly of the Cu center of NOR, and thus represents the first characterised assembly factor for the Cu active site of this key environmental enzyme, which is globally responsible for the destruction of a potent greenhouse gas.