AI Article Synopsis
- The study focused on the detection of Gram-negative anaerobic bacteria in dogs' intestines for the first time in Iran, analyzing fecal samples from both healthy and diarrheic dogs.
- A total of 151 samples were obtained, revealing that 9.27% contained spirochaetes, confirmed by specific gene testing.
- The results indicated a higher prevalence of these bacteria in younger dogs, with a significant association between age and detection rates, and suggested greater genetic variability in the targeted gene of the isolated bacteria.
Article Abstract
Genus , as Gram negative anaerobic bacteria, colonize in dogs intestine. The aim of the current study was to determine the prevalence of spp. for the first time in Iran and rapid identification of spp. in dogs by a new designment of a species-specific primer set for . One hundred fifty-one fecal samples were obtained from dogs by rectal swab. Twenty dogs suffered from diarrhea and 131 of them were healthy. In 9.27% (14/151) of samples, spirochaetes were detected on primary cultures by weak hemolysis and positive Gram staining and then genus was confirmed by NADH oxidase () gene via polymerase chain reaction. Among 14 isolates, twelve isolates were , one isolate was and another one was non-typeable. From 12 , only eight isolates were detected by designed specific primers. Ten spp. were isolated from dogs ≤ 1 year old (10/67, 14.92%) and 4 isolates were from > 1 year old dogs (4/84, 4.76%). The isolation rates from healthy and diarrheic dogs were (12/131, 9.16%) and (2/20, 10.00%), respectively. A statistically significant association was observed between the presence of spp. and the age under one year. Based on our findings, the gene in might have more sequence variability compared to other spp.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6522192 | PMC |
| http://dx.doi.org/10.30466/vrf.2019.34309 | DOI Listing |
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