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Addendum: Precise tuning of gene expression levels in mammalian cells. | LitMetric

AI Article Synopsis

Article Abstract

Following re-sequencing of the miSFIT constructs used in the paper, two of the construct variants inserted into the 3'UTR of PD-1, namely '12C' and '17A, 18G', have been found to contain additional insertions not present in the other construct variants. The data points corresponding to these constructs in Figs. 2c, f and Supplementary Fig. 9 are therefore no longer valid. However the overall conclusion that step-wise control over gene expression levels using the miSFIT constructs remains unaffected by these errors. Updated versions of Fig. 2 and Supplementary Fig. 9 are presented in the accompanying Addendum.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6557827PMC
http://dx.doi.org/10.1038/s41467-019-10615-0DOI Listing

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