AI Article Synopsis
- Researchers studied how two types of dye-conjugated cucurbit[7]urils (dye-CB[7]), used as molecular probes for live cell imaging, are taken up and expelled by live cells, specifically MCF-7 human breast carcinoma cells.
- The dye-CB[7]s enter cells mainly through clathrin-mediated endocytosis and are excreted via lysosome-associated exocytosis.
- The structure of the CB[7] plays a significant role in determining these uptake and excretion pathways, suggesting potential for developing new imaging probes.
Article Abstract
Here we report the endocytosis and excretion pathways of two different dye-conjugated cucurbit[7]urils, (cyanine 3-conjugated CB[7] and rhodamine X-conjugated CB[7]), which have great potential as molecular probes for live cell imaging. The dye-CB[7]s are translocated into live cells (human breast carcinoma cells, MCF-7) via multiple pathways, predominantly by clathrin-mediated endocytosis, and excreted from cells via lysosome-associated exocytosis. Interestingly, the CB[7] moiety has a substantial influence on the uptake and excretion pathways. These findings may widen the applications of the dyes conjugated to CB[7] and assist in the design of new molecular probes for live cell imaging.
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| http://dx.doi.org/10.1039/c9ob00356h | DOI Listing |
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