The development of pesticide resistance in insects and recent bans on pesticides call for the identification of natural sources of resistance in crops. Here, we used natural variation in pepper (Capsicum spp.) resistance combined with an untargeted metabolomics approach to detect secondary metabolites related to thrips (Frankliniella occidentalis) resistance. Using leaf disc choice assays, we tested 11 Capsicum accessions of C. annuum and C. chinense in both vegetative and flowering stages for thrips resistance. Metabolites in the leaves of these 11 accessions were analyzed using LC-MS based untargeted metabolomics. The choice assays showed significant differences among the accessions in thrips feeding damage. The level of resistance depended on plant developmental stage. Metabolomics analyses showed differences in metabolomes among the Capsicum species and plant developmental stages. Moreover, metabolomic profiles of resistant and susceptible accessions differed. Monomer and dimer acyclic diterpene glycosides (capsianosides) were pinpointed as metabolites that were related to thrips resistance. Sucrose and malonylated flavone glycosides were related to susceptibility. To our knowledge, this is the first time that dimer capsianosides of pepper have been linked to insect resistance. Our results show the potential of untargeted metabolomics as a tool for discovering metabolites that are important in plant - insect interactions.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6570690 | PMC |
| http://dx.doi.org/10.1007/s10886-019-01074-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
RNA sequencing of Beauveria bassiana JEF-350-infected Thrips palmi reveals change of host defense and homeostasis.
Appl Microbiol Biotechnol
November 2024
Department of Agricultural Biology, College of Agriculture & Life Sciences, Jeonbuk National University, Jeonju, South Korea.
Melon thrips, Thrips palmi, represent a significant threat to plants, inducing necrosis and acting as vectors for numerous plant viruses. Entomopathogenic fungi present a promising avenue for the management of melon thrips populations resistant to conventional chemical treatments. In this work, an adult colony of melon thrips was exposed to Beauveria bassiana strain JEF-350, and the ensuing transcriptional response of the infected thrips was scrutinized to elucidate their reactions during fungal pathogenesis.
View Article and Find Full Text PDFSpraying calcium chloride helps to enhance the resistance of kidney bean plants to western flower thrips.
Pest Manag Sci
January 2025
Institute of Entomology, Guizhou University, The Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Guiyang, China.
Background: The western flower thrips (WFT), Frankliniella occidentalis (Thysanoptera: Thripidae), is a significant pest in horticulture and ornamental agriculture. While exogenous calcium (Ca) has been shown to confer plant immune responses against thrips, the detailed mechanisms of this interaction remain to be elucidated for improved thrips management strategies. This study aimed to assess the impact of exogenous Ca on WFT feeding behavior and to explore its role in enhancing the defense mechanisms of kidney bean plants against WFT attacks.
View Article and Find Full Text PDFChromosome-level genome assembly of cotton thrips Thrips tabaci (Thysanoptera: Thripidae).
Sci Data
September 2024
State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, China.
Cotton thrip, Thrips tabaci is a major polyphagous pest widely distributed on a variety of crops around the world, causing huge economic losses to agricultural production. Due to its biological and genomic characteristics, this pest can reproduce quickly and develop resistance to various pesticides in a very short time. However, the lack of high-quality reference genomes has hindered deeper gene function exploration and slows down the development of new management strategies.
View Article and Find Full Text PDFIdentification and analysis of JHBP/TO family genes and their roles in the reproductive fitness cost of resistance in Frankliniella occidentalis (Pergande).
Pestic Biochem Physiol
September 2024
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100080, China. Electronic address:
The juvenile hormone binding protein (JHBP) and takeout (TO) genes, mediated by the juvenile hormone (JH), play a crucial role in regulating the reproductive physiology of insects. Our previous study revealed that spinosad-resistant Frankliniella occidentalis (NIL-R) exhibited reduced fecundity and significant changes in JHBP/TO family gene expression. We hypothesized that these genes were involved in regulating the fitness costs associated with resistance.
View Article and Find Full Text PDFGenome-wide investigation of the TIFY transcription factors in alfalfa (Medicago sativa L.): identification, analysis, and expression.
BMC Plant Biol
September 2024
Technology Engineering Center of Drought and Cold-Resistant Grass Breeding in North of the National Forestry and Grassland Administration, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, China.
Background: Alfalfa (Medicago sativa L.) is an essential leguminous forage with high nutrition and strong adaptability. The TIFY family is a plant-specific transcription factor identified in many plants.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!