AI Article Synopsis
- Biofilms are formed by bacteria like Streptococcus mutans and Aggregatibacter actinomycetemcomitans, generating extracellular polymeric substances (EPS) influenced by components such as sucrose and soy protein.
- A study aimed to identify the specific molecular weight that affects the protein profiles of these bacteria's biofilms in the presence of sucrose and soy protein.
- Results showed varying numbers of protein bands for each bacterial biofilm under different media conditions, emphasizing the differences in molecular weight and biofilm protein profiles between the two species.
Article Abstract
Context: Biofilms consist of microbial cells and extracellular polymeric substance (EPS). Streptococcus mutans and Aggregatibacter actinomycetemcomtans are bacteria that can form biofilms and generate EPS. Biofilm formation can be induced by specific substances such as sucrose and protein.
Aims: To identify the molecular weight that determines biofilm protein profile expression of S. mutans and A. actinomycetemcomitans induced by sucrose (carbohydrate) and soy protein (glycine soja).
Settings And Design: Experimental laboratory study.
Materials And Methods: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to determine the molecular weight.
Statistical Analysis Used: Nil.
Results: The results of analysis of protein SDS-PAGE showed the presence of 28 protein bands on A. actinomycetemcomitans biofilm in the media trypticase soy broth (TSB), 20 protein bands on biofilms of S. mutans in the media TSB, 29 protein bands on biofilm A. actinomycetemcomitans in the media brain heart infusion (BHI) + sucrose 2%, and 13 protein bands on biofilms of S. mutans in the media BHI + sucrose 2%.
Conclusion: There are differences in biofilm protein profile expression that determine the molecular weight of S. mutans biofilm and A. actinomycetemcomitans induced by sucrose (carbohydrate) and soy protein (glycine soja).
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.4103/ijdr.IJDR_183_17 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Detection of Mycobacterium tuberculosis DNA in intraocular fluid of 11 suspected tuberculous uveitis patients by multiplex PCR.
BMC Ophthalmol
January 2025
Department of Tuberculosis, New District Branch of Northern Jiangsu People's Hospital of Jiangsu Province, Yangzhou, 225001, Jiangsu Province, China.
Background: This study aims to detect Mycobacterium tuberculosis complex (MTBC) DNA in intraocular fluid from clinically suspected tuberculous uveitis patients using multiplex polymerase chain reaction (PCR) and investigate the diagnostic utility of multiplex PCR for tuberculous uveitis.
Methods: Primers targeting three specific genes (MPB64, CYP141, and IS6110) within the MTBC genome were designed. Multiplex PCR was conducted using DNA from the H37Rv strain as well as DNA extracted from fluids of confirmed tuberculosis patients to assess primer specificity and method feasibility.
Potential mutagenicity of aflatoxin B1 in Egyptian spices.
BMC Genomics
January 2025
Botany Department, Faculty of Science, Mansoura University, Mansoura, Egypt.
The current study aimed to detect the mutagenic impacts of aflatoxin B1 (AFB1), which is produced by Aspergillus group fungi, via a high-plant genotoxicity test. Different durations of treatment (3 h, 6 h, and 12 h) were used to treat the Vicia faba root tips with varying concentrations of Aflatoxin B1 (AFB1) following the approved protocol for plant assays published by the International Program on Chemical Safety (IPCS) and the World Health Organization (WHO). The data obtained indicated that AFB1 not only has the ability to induce various alterations in the process of mitosis, ranging from increasing to decreasing mitotic and phase indices but also leads to many mitotic aberrations.
View Article and Find Full Text PDF[Retracted] Differential effects of PXD101 (belinostat) on androgen-dependent and androgen-independent prostate cancer models.
Int J Oncol
February 2025
Department of Experimental Medicine, Laboratory of Radiobiology, University of L'Aquila, I-67100 L'Aquila, Italy.
Article Synopsis
- A reader noticed that some data in a published article closely resembled data from a previous study by different authors, raising concerns about potential plagiarism.
- An independent review confirmed the presence of duplicated protein bands and reused data in the figures of the article.
- The Editor ultimately decided to retract the paper, with the corresponding author accepting responsibility while stating that other co-authors were not involved in creating the figures.
Discovery of Titin and Its Role in Heart Function and Disease.
Circ Res
January 2025
Department of Integrative Pathophysiology, Medical Faculty Mannheim, DZHK Partnersite Mannheim-Heidelberg, University of Heidelberg, Germany (S.L.).
This review examines the giant elastic protein titin and its critical roles in heart function, both in health and disease, as discovered since its identification nearly 50 years ago. Encoded by the TTN (titin gene), titin has emerged as a major disease locus for cardiac disorders. Functionally, titin acts as a third myofilament type, connecting sarcomeric Z-disks and M-bands, and regulating myocardial passive stiffness and stretch sensing.
View Article and Find Full Text PDFSurface-enhanced Raman spectroscopy for the characterization of filtrate portions of blood serum samples of myocardial infarction patients using 30 kDa centrifugal filter devices.
Spectrochim Acta A Mol Biomol Spectrosc
December 2024
Department of Chemistry, Institut - Courtois, Quebec Center for Advanced Materials (QCAM), and Regroupement Québécois sur les Matériaux de Pointe (RQMP), Université de Montréal, Montréal, Quebec H3C 3J7, Canada.
Myocardial infarction (MI) is the leading cause of death and disability worldwide. It occurs when a thrombus forms after an atherosclerotic plaque bursts, obstructing blood flow to the heart. Prompt and accurate diagnosis is crucial for improving patient survival.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!