The metabolism of aflatoxin B1 by the post-mitochondrial fraction of human autopsy or biopsy liver and monkey necropsy liver was investigated. Aflatoxin Q1, a hydroxy metabolite of B1, was produced by 18 of 22 human liver samples and by 5 of 7 species of non-human primates investigated. Human specimens from both sexes, ages 20-80 years, with a variety of hepatic histological and historical influences produced this metabolite. Aflatoxin Q1 was of variable quantitative importance. Yields seldom exceeded 10% of initial B1. However, based upon its persistent formation in vitro, these studies identify the need to clarify the role of Q1 in aflatoxin epidemiology.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0378-4274(87)90002-6 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Bafilomycin A1 Molecular Effect on ATPase Activity of Subcellular Fraction of Human Colorectal Cancer and Rat Liver.
Int J Mol Sci
January 2024
Department of Experimental Biology, Faculty of Science, Masaryk University, 625 00 Brno, Czech Republic.
Bafilomycin A1 inhibits V-type H ATPases on the molecular level, which acidifies endo-lysosomes. The main objective of the study was to assess the effect of bafilomycin A1 on Ca content, NAADP-induced Ca release, and ATPase activity in rat hepatocytes and human colon cancer samples. Chlortetracycline (CTC) was used for a quantitative measure of stored calcium in permeabilized rat hepatocytes.
View Article and Find Full Text PDFMitoquinol mesylate (MITOQ) attenuates diethyl nitrosamine-induced hepatocellular carcinoma through modulation of mitochondrial antioxidant defense systems.
Toxicol Res
July 2022
Department of Anatomic and Molecular Pathology, Faculty of Basic Medical Sciences,, College of Medicine of University of Lagos, Idi-araba, P.M.B. 12003 Lagos, Nigeria.
Diethyl nitrosamine (DEN) induced cirrhosis-hepatocellular carcinoma (HCC) model associates cancer progression with oxidative stress and mitochondrial dysfunction. This study investigated the effects of mitoquinol mesylate (MitoQ), a mitochondrial-targeted antioxidant on DEN-induced oxidative damage in HCC Wistar rats. Fifty male Wistar rats were randomly divided into five groups.
View Article and Find Full Text PDFApplication of Non-Destructive Methods: Biomarker Assays in Blood of White Stork () Nestlings.
Animals (Basel)
August 2021
Department of Biology, Josip Juraj Strossmayer University of Osijek, 31000 Osijek, Croatia.
White stork () nestlings can provide quantitative information on the quality of the surrounding environment by indicating the presence of pollutants, as they depend on locally foraged food. This study represents the first comparison of biomarkers in two fractions of white stork nestling blood: plasma and S9 (the post-mitochondrial fraction). The aim of this study was to evaluate acetylcholinesterase (AChE), carboxylesterase (CES), glutathione S-transferase (GST), and glutathione reductase (GR), as well as to establish a novel fluorescence-based method for glutathione (GSH) and reactive oxygen species (ROS) detection in plasma and S9.
View Article and Find Full Text PDFINFLUENCE OF ARTESUNATE COMBINATIVE THERAPY CO-ADMINISTRATION WITH RUTIN ON INFLAMMATORY CYTOKINES AND IMMUNOGLOBULINS IN PLASMODIUM BERGHEI-INFECTED MICE.
J Parasitol
July 2021
Laboratories for Biomembrane Research and Biotechnology, Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Nigeria 200001.
Some antimalarial drugs are immune-modulators that impact multiple pathways of innate immunity in malarial treatment. However, information on the immunomodulatory effects of artequine and rutin in the treatment of malaria remains elusive. Twenty-five Swiss mice (18 ± 2 g) were used for this study.
View Article and Find Full Text PDFIn vitro metabolism of lidocaine in subcellular post-mitochondrial fractions and precision cut slices from cattle liver.
Toxicol In Vitro
October 2021
Wageningen Food Safety Research, Wageningen University and Research, PO Box 230, 6700 AE Wageningen, the Netherlands.
In vitro models are widely used to study the biotransformation of xenobiotics and to provide input parameters to physiologically based kinetic models required to predict the kinetic behavior in vivo. For farm animals this is not common practice yet. The use of slaughterhouse-derived tissue material may provide opportunities to study biotransformation reactions in farm animals.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!