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Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases. | LitMetric
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Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases.

Kun Huang Fabio Parmeggiani Helene Ledru Kristian Hollingsworth Jordi Mas Pons Andrea Marchesi Peter Both Ashley P Mattey Edward Pallister Gregory S Bulmer Jolanda M van Munster W Bruce Turnbull M Carmen Galan Sabine L Flitsch

Org Biomol Chem

Manchester Institute of Biotechnology (MIB), School of Chemistry, The University of Manchester, 131 Princess Street, M1 7DN, Manchester, UK.

Published: June 2019

Utilising a fast and sensitive screening method based on imidazolium-tagged probes, we report unprecedented reversible activity of bacterial β1,4-galactosyltransferases to catalyse the transgalactosylation from lactose to N-acetylglucosamine to form N-acetyllactosamine in the presence of UDP. The process is demonstrated by the preparative scale synthesis of pNP-β-LacNAc from lactose using β1,4-galactosyltransferase NmLgtB-B as the only biocatalyst.

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 Source
http://dx.doi.org/10.1039/c9ob01089kDOI Listing

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