(1) Background: Heme oxygenase-1 (HO-1) degrades heme and generates carbon monoxide (CO), producing various anti-inflammatory, anti-oxidative, and anti-apoptotic effects. This study aimed to confirm the effects of CO on the ischemia-reperfusion injury (IRI) of donor lungs using a high-pressure gas (HPG) preservation method. (2) Methods: Donor rat and canine lungs were preserved in a chamber filled with CO (1.5 atm) and oxygen (O; 2 atm) and were ventilated with either CO and O mixture (CO/O group) or air (air group) immediately before storage. Rat lungs were subjected to heterotopic cervical transplantation and evaluated after reperfusion, whereas canine lungs were subjected to allogeneic transplantation and evaluated. (3) Results: Alveolar hemorrhage in the CO/O group was significantly milder than that in the air group. mRNA expression levels of HO-1 remained unchanged in both the groups; however, inflammatory mediator levels were significantly lower in the CO/O group than in the air group. The oxygenation of graft lungs was comparable between the two groups, but lactic acid level tended to be higher in the air group. (4) Conclusions: The HO-1/CO system in the HPG preservation method is effective in suppressing IRI and preserving donor lungs.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600409PMC
http://dx.doi.org/10.3390/ijms20112719DOI Listing

Publication Analysis

Top Keywords

air group
16
co/o group
12
carbon monoxide
8
donor lungs
8
hpg preservation
8
preservation method
8
canine lungs
8
group air
8
lungs subjected
8
transplantation evaluated
8

Similar Publications

Background Obstructive sleep apnea (OSA) is an intermittent hypoxia disorder associated with cognitive dysfunction, including learning and memory impairments. There is evidence that alterations in protease activity and neuronal activation as associated with cognitive dysfunction, are dependent on sex, and may be brain region-specific. However, the mechanisms mediating OSA-induced cognitive impairments are unclear.

View Article and Find Full Text PDF

Background: Spatial repellent products are used for prevention of insect bites, and a body of evidence exists on spatial repellent entomological efficacy. A new option for vector control, spatial repellent products are designed to release active ingredient into the air for disruption of human-vector contact thereby reducing human exposure to mosquito-borne pathogens. Clinical trials have shown spatial repellent epidemiological efficacy against Aedes-borne viruses but inconclusive outcomes against malaria.

View Article and Find Full Text PDF

Exposure of cell cultures at air-liquid interface (ALI), mimicking i.e. human lung surface, is believed to be one of the most realistic means to model toxicity of complex mixtures of pollutants on human health.

View Article and Find Full Text PDF

Microbiology reference laboratories perform a crucial role within public health systems. This role was especially evident during the COVID-19 pandemic. In this Viewpoint, we emphasise the importance of microbiology reference laboratories and highlight the types of digital data and expertise they provide, which benefit national and international public health.

View Article and Find Full Text PDF

Colour Changes and Surface Roughness After Air-Polishing for Tobacco Stain Removal.

Int Dent J

December 2024

Department of Dental and Oral Medicine and Cranio-maxillofacial and Oral Surgery, University Hospital for Conservative Dentistry and Periodontology, Medical University of Innsbruck, Innsbruck, Austria.

Introduction And Aims: We aimed to investigate the efficacy of air-polishing in restoring the original tooth colour of standardised tobacco-stained tooth specimens.

Methods: Seventy-two specimens consisting of half dentine and half enamel were daily exposed to the smoke of five cigarettes in an automated smoking chamber. Four repetitions of a 14-day smoking cycle were performed.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!