AI Article Synopsis
- Japanese encephalitis virus SA14-14-2 is a vaccine used to prevent the disease in children across China and Southeast Asia.
- A stable infectious cDNA clone of JEV SA14-14-2 was created using a low copy number vector, incorporating introns for gene stability and a ribozyme for proper transcription.
- This research establishes a reverse genetics system for JEV SA14-14-2, which could aid in developing other vaccines and studying viral pathogenesis.
Article Abstract
Japanese encephalitis virus SA14-14-2 (JEV SA14-14-2) is a widely used vaccine in China and other southeastern countries to prevent Japanese encephalitis in children. In this study, a stable infectious cDNA clone of JEV SA14-14-2 with a low copy number pACYC177 vector dependent on the T7 promoter and T7 terminator was developed. Two introns were inserted into the capsid gene and envelope gene of JEV cDNA for gene stability. Hepatitis delta virus ribozyme (HDVr) was engineered into the 3' UTR cDNA of JEV for authentic 3' UTR transcription. The rescued virus showed biological properties indistinguishable from those of the parent strain (JEV SA14-14-2). The establishment of a JEV SA14-14-2 reverse genetics system lays the foundation for the further development of other flavivirus vaccines and viral pathogenesis studies.
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| http://dx.doi.org/10.1007/s11262-019-01674-y | DOI Listing |
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