Dual control of RegX3 transcriptional activity by SenX3 and PknB.

The mycobacterial SenX3-RegX3 two-component system consists of the SenX3 sensor histidine kinase and its cognate RegX3 response regulator. This system is a phosphorelay-based regulatory system involved in sensing environmental P levels and induction of genes required for P acquisition under P-limiting conditions. Here we demonstrate that overexpression of the kinase domain of PknB (PknB-KD) inhibits the transcriptional activity of RegX3 of both and (RegX3 and RegX3, respectively). Mass spectrometry results, along with those of phosphorylation and complementation analyses, revealed that PknB kinase activity inhibits the transcriptional activity of RegX3 through phosphorylation events at Thr-100, Thr-191, and Thr-217. Electrophoretic mobility shift assays disclosed that phosphorylation of Thr-191 and Thr-217 abolishes the DNA-binding ability of RegX3 and that Thr-100 phosphorylation likely prevents RegX3 from being activated through conformational changes induced by SenX3-mediated phosphorylation. We propose that the convergence of the PknB and SenX3-RegX3 signaling pathways might enable mycobacteria to integrate environmental P signals with the cellular replication state to adjust gene expression in response to P availability.