In a previous study, mutants with enhanced ciprofloxacin resistance (Cip) were selected from J53/pMG252 carrying Strain J53 Cip 8-2 showed an increase in the copy number and transcription level of We sequenced the plasmids on Illumina and MinION platforms. Parental plasmid pMG252 and plasmid pMG252A from strain J53 Cip 8-2 were almost identical, except for the region containing that in pMG252A contained 4 additional copies of the Δ-IS region.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6658789PMC
http://dx.doi.org/10.1128/AAC.00718-19DOI Listing

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