The detailed investigation of methylene blue (MB) dye with ovalbumin (OVA) was examined by multispectroscopic and computational techniques. The experimental results of emission spectral studies displayed that the quenching behaviour of OVA with MB dye is due to static quenching mechanism. Isothermal titration calorimetry experimental results suggested that the binding of MB dye became favoured with the aid of a favourable entropy contribution and negative enthalpy. The absorption and circular dichroism spectral experiments showed that the binding of MB dye prompted conformational modifications to the secondary structure of OVA protein. The computational studies have been used to predict the binding region and the stability of MB in OVA protein.Communicated by Ramaswamy H. Sarma.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/07391102.2019.1618734 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Acquired methemoglobinemia in infancy secondary to diarrhea: a case report.
Pan Afr Med J
September 2024
Al Jalila Children's Hospital, Dubai Health, Dubai, United Arab Emirates.
Methemoglobinemia (MetHb) is a life-threatening condition that reduces the oxygen-carrying ability of hemoglobin. Acquired methemoglobinemia usually results from exposure to specific oxidizing agents. Symptoms and complications depend on the MetHb level, which can sometimes be fatal.
View Article and Find Full Text PDFAn electrochemical aptasensor for the detection of bisphenol A based on triple signal amplification assisted by gold nanoparticles, hemin/G-quadruplex DNAzyme, and exonuclease I.
Mikrochim Acta
December 2024
Key Laboratory for Analytical Science of Food Safety and Biology, MOE, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350108, China.
A triple signal amplified electrochemical aptasensor for the detection of bisphenol A (BPA) was developed for the first time based on gold nanoparticles (AuNPs), hemin/G-quadruplex DNAzyme, and exonuclease I (Exo I) assisted amplification strategies. The BPA aptamer (Apt) hybridized with the capture probe (CP) was fixed on the gold electrode (GE) to form the double-stranded DNA (dsDNA) structure. When BPA was present, the Apt was detached from the GE surface by specific recognition between the BPA and Apt, forming BPA-Apt complexes in solution.
View Article and Find Full Text PDF[Modified endoscopic mucosal resection for the treatment of early gastrointestinal lesions].
Zhonghua Wei Chang Wai Ke Za Zhi
December 2024
Department of Gastroenterology, Dongguan Children's Hospital Affiliated to Guangdong Medical University, Dongguan Eighth People's Hospital, Dongguan523000, China.
To evaluate the clinical efficacy of modified endoscopic mucosal resection (EMR) in the management of early gastrointestinal lesions. Upon endoscopic identification of the lesion, normal saline is injected into the submucosa to establish a fluid cushion, which elevates the lesion's mucosa. Methylene blue may also be applied to improve visualization of the relationship between the submucosa and muscular layer.
View Article and Find Full Text PDFAbatement of methylene blue and diazinon pesticide from synthetic solutions using magnetic biochar from pistachio shells modified with MOF-808.
Environ Res
December 2024
Department of Civil Engineering, College of Engineering, King Saud University, P.O. Box 800, Riyadh, 11421, Saudi Arabia.
This study develops a magnetic composite from pistachio shell biochar (PSBC/CoFe₂O₄) modified with MOF-808 for removing methylene blue (MB) dye and diazinon (DA) pesticide from water. The composite, with a surface area of 151.53 m/g and magnetic saturation of 19.
View Article and Find Full Text PDFβ-cyclodextrin imprinted film embedded with methylene blue: A host-guest sensitive electrochemical strategy for PFAS detection.
J Hazard Mater
December 2024
State Key Laboratory of Pollution Control and Resources Reuse (Tongji University), College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China. Electronic address:
Per- and polyfluoroalkyl substances (PFAS) have raised significant concerns; however, their accurate detection in aqueous environments remains a major challenge. In this study, a host-guest molecularly imprinted polymer-based electrochemical sensor with enhanced antifouling properties were developed using β-cyclodextrin embedded with methylene blue (βCD-MB MIP). This sensor demonstrated sensitive and selective quantification of perfluorooctanoic acid (PFOA) in real water samples.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!