An indirect enzyme-linked immunosorbent assay (ELISA) has been developed for the identification of smoked meat from salmon ( Salmo salar ), trout ( Oncorhynchus mykiss ), and bream ( Erama raii ). The assay uses polyclonal antibodies raised in rabbits against soluble proteins of muscle from salmon (anti-SSP), trout (anti-TSP), and bream (anti-BSP) which are rendered species-specific by blocking them with the heterologous soluble muscle proteins. The blocked antibodies were used to detect the samples from smoked fish bound to the wells of a microtiter plate. Immunorecognition of polyclonal antibodies adsorbed to fish samples was made with goat anti-rabbit immunoglobulins conjugated to the enzyme horseradish peroxidase. Subsequent enzymic coversion of the substrate allowed clear species identification of smoked meat of salmon, trout, and bream.
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