Immunostick ELISA for Detection of Cow's Milk in Ewe's Milk and Cheese Using a Monoclonal Antibody against Bovine β-Casein.

J Food Prot

Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.

Published: April 1996

AI Article Synopsis

  • An immunostick ELISA was created to quickly detect cow's milk in ewe's milk or cheese.
  • It utilizes a monoclonal antibody against bovine β-casein to recognize the presence of cow's milk.
  • This method enables visible identification of ewe's milk with over 1% cow's milk or cheese samples with over 0.5% cow's cheese.

Article Abstract

An immunostick enzyme-linked immunosorbent assay (ELISA) has been developed for the rapid detection of cow's milk in ewe's milk or cheese. The assay uses a monoclonal antibody (AH4) produced against bovine β-casein for the detection of cow's milk or cheese bound to the paddles of immunostick tubes. This immunostick ELISA allows the visual identification of ewe's milk containing more than 1% of cow's milk or cheese samples containing more than 0.5% of cow's cheese.

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Source
http://dx.doi.org/10.4315/0362-028X-59.4.436DOI Listing

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