ATCC11845 (RA ATCC11845) is naturally competent. However, the genes involved in natural transformation in this species remain largely unknown. Bioinformatic analysis predicts that DprA of RA (DprA) has three domains: a sterile alpha motif (SAM), a Rossmann fold (RF) domain and a Z-DNA-binding domain (Zα). Inactivation of abrogated natural transformation in RA ATCC11845, and this effect was restored by the expression of . The with SAM and RF domains of and the with RF and Zα domains of was able to restore natural transformation in the RA ATCC11845 mutant. An Arg123 mutation in the RF domain of was not able to restore natural transformation of the RA ATCC11845 mutant. Furthermore, DprA abolished its ability to bind DNA, suggesting that the RF domain is essential for the function of DprA. Finally, the of which has not been reported to be natural competent currently was partially able to restore natural transformation in RA ATCC11845 mutant. These results collectively suggest that DprA has a conserved evolutionary mechanism.
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| http://dx.doi.org/10.3389/fgene.2019.00429 | DOI Listing |
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