Severity: Warning
Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
Filename: helpers/my_audit_helper.php
Line Number: 176
Backtrace:
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3122
Function: getPubMedXML
File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global
File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword
File: /var/www/html/index.php
Line: 316
Function: require_once
is increasingly recognized as a potent pathogen, responsible for severe infections with an outcome resembling that of . Here, we developed and evaluated a tool for typing, using DNA sequence analysis of the repeat-encoding region (R-domain) in the gene encoding the fibrinogen (Fg)-binding protein Fbl (-typing). We typed 240 isolates from various clinical and geographical origins. The length of the R-domain ranged from 9 to 52 repeats. -typing identified 54 unique 18-bp repeat sequences and 92 distinct -types. The discriminatory power of -typing was higher than that of multilocus sequence typing (MLST) and equivalent to that of tandem repeat sequence typing. -types could assign isolates to MLST clonal complexes with excellent predictive power. The ability to promote adherence to immobilized human Fg was evaluated for 55 isolates chosen to reflect the genetic diversity of the gene. We observed no direct correlation between Fg binding ability and -types. However, the lowest percentage of Fg binding was observed for isolates carrying a 5'-end frameshift mutation of the gene and for those harboring fewer than 43 repeats in the R-domain. qRT-PCR assays for some isolates revealed no correlation between gene expression and Fg binding capacity. In conclusion, this study shows that -typing is a useful tool in epidemiology, especially because it is an easy, cost-effective, rapid and portable method (http://fbl-typing.univ-rouen.fr/). The impact of polymorphism on the structure of the protein, its expression on the cell surface and in virulence remains to be determined.
Download full-text PDF |
Source |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6533592 | PMC |
http://dx.doi.org/10.3389/fmicb.2019.01109 | DOI Listing |
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