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-Typing of : A Frontline Tool for Epidemiological Studies, but Not Predictive of Fibrinogen Binding Ability. | LitMetric

is increasingly recognized as a potent pathogen, responsible for severe infections with an outcome resembling that of . Here, we developed and evaluated a tool for typing, using DNA sequence analysis of the repeat-encoding region (R-domain) in the gene encoding the fibrinogen (Fg)-binding protein Fbl (-typing). We typed 240 isolates from various clinical and geographical origins. The length of the R-domain ranged from 9 to 52 repeats. -typing identified 54 unique 18-bp repeat sequences and 92 distinct -types. The discriminatory power of -typing was higher than that of multilocus sequence typing (MLST) and equivalent to that of tandem repeat sequence typing. -types could assign isolates to MLST clonal complexes with excellent predictive power. The ability to promote adherence to immobilized human Fg was evaluated for 55 isolates chosen to reflect the genetic diversity of the gene. We observed no direct correlation between Fg binding ability and -types. However, the lowest percentage of Fg binding was observed for isolates carrying a 5'-end frameshift mutation of the gene and for those harboring fewer than 43 repeats in the R-domain. qRT-PCR assays for some isolates revealed no correlation between gene expression and Fg binding capacity. In conclusion, this study shows that -typing is a useful tool in epidemiology, especially because it is an easy, cost-effective, rapid and portable method (http://fbl-typing.univ-rouen.fr/). The impact of polymorphism on the structure of the protein, its expression on the cell surface and in virulence remains to be determined.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6533592PMC
http://dx.doi.org/10.3389/fmicb.2019.01109DOI Listing

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