Background: The study aimed to investigate whether timolol-treatment has a beneficial effect on pentose phosphate pathway enzyme activities such as glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGDH) enzyme activities and cAMP level in streptozotocin-induced diabetic rats in pancreatic tissues.
Methods: Diabetes was induced by streptozotocin (STZ) in 3-month old male Wistar rats. The diabetic rats were treated with timolol (5 mg/kg body weight, for 12 weeks) while the control group received saline. Enzyme activities were determined in pancreas tissue. To support our results, we performed calculations, using Protein Data Bank structures.
Results: Timolol treatment of STZ-induced diabetic rats had no noteworthy effect on high blood-glucose levels. However, this treatment induced activities of G6PD and 6PGDH in diabetic rats. Timolol treatment significantly increased cAMP level in diabetic pancreatic tissue. We found that timolol cannot bind strongly to either G6PD or 6PGD, but there is a relatively higher binding affinity to adenylyl cyclase, responsible for cAMP production, serving as a regulatory signal via specific cAMP-binding proteins.
Conclusions: Our data point out that timolol treatment has beneficial effects on the antioxidant defence mechanism enzymes in the pancreas of STZ-induced diabetic rats.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6534949 | PMC |
| http://dx.doi.org/10.2478/jomb-2018-0034 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A truncated isoform of Connexin43 caps actin to organize forward delivery of full-length Connexin43.
J Cell Biol
March 2025
Nora Eccles Harrison Cardiovascular Research and Training Institute, University of Utah, Salt Lake City, UT, USA.
While membrane proteins such as ion channels continuously turn over and require replacement, the mechanisms of specificity of efficient channel delivery to appropriate membrane subdomains remain poorly understood. GJA1-20k is a truncated Connexin43 (Cx43) isoform arising from translation initiating at an internal start codon within the same parent GJA1 mRNA and is requisite for full-length Cx43 trafficking to cell borders. GJA1-20k does not have a full transmembrane domain, and it is not known how GJA1-20k enables forward delivery of Cx43 hemichannels.
View Article and Find Full Text PDFBeneficial Effects of Tilapia Fish Skin on Excisional Skin Wound Healing in a Type I Diabetic Rat Model.
J Biomed Mater Res B Appl Biomater
January 2025
Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt.
IntroductionProlonged hyperglycemia in diabetic patients often impairs wound healing, leading to chronic infections and complications. This study aimed to evaluate the potential of fresh Tilapia fish skin as a treatment to enhance wound healing in diabetic rats. MethodsThirty-nine healthy adult albino rats, weighing between 150 and 200 g, were divided into three groups: non-diabetic rats with untreated wounds [C-], diabetic rats with untreated wounds [C+], and diabetic rats treated with fresh Tilapia skin [TT].
View Article and Find Full Text PDFBroccoli () leaves exhibit significant antidiabetic potential in alloxan-induced diabetic rats: the putative role of ABC vacuolar transporter for accumulation of Quercetin and Kaempferol.
Front Pharmacol
December 2024
Department of Biochemistry, Quaid-i-Azam University, Islamabad, Pakistan.
Background: The global prevalence of diabetes among adults over 18 years of age is expected to increase from 10.5% to 12.2% (between 2021 and 2045).
View Article and Find Full Text PDFEffect of leaf extract and its active components on heart muscle cell apoptosis induced by hyperglycemia.
Open Vet J
November 2024
Department of Anatomical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
Background: Hyperglycemia is a condition in which blood sugar levels increase excessively due to a variety of factors, one of which is the body's inability to regulate insulin properly. Diabetes closely relates to this condition, which significantly contributes to premature death and disability. Long-term diabetes treatment accompanied by a strict diet provides real results in controlling blood glucose levels but can cause side effects.
View Article and Find Full Text PDFInvolvement of γ-Aminobutyric Acid and N-methyl-D-aspartate Receptors in Diabetic Gastropathy in Rats: Possible Beneficial Effect of Prolonged Treatment with Insulin and Magnesium Supplement.
Arch Razi Inst
June 2024
Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Gastrointestinal dysfunction is a severe and common complication in diabetic patients. Some evidence shows that gamma-aminobutyric acid (GABA) and glutamate contribute to diabetic gastrointestinal abnormalities. Therefore, we examined the impact of prolonged treatment with insulin and magnesium supplements on the expression pattern of GABA type A (GABA-A), GABA-B, and N-methyl-D-aspartate (NMDA) glutamate receptors as well as nitric oxide synthase 1 (NOS-1) in the stomach of type 2 diabetic rats.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!